↓ Skip to main content
Nacef Guessaymi

Publications

Journal Article 2026

Live-cell 3D mapping reveals the organized architecture of FG-nucleoporins within the nuclear pore complex

Yu W., Kelich J., Yu J., Tingey M., Li Y., Junod S., Jiang Z., Hansen I., Guessaymi N., Yang W.

Nature Communications (Article in Press)

DOI

Live-cell single-molecule tracking with 2D-to-3D reconstruction, cross-validated by 3D MINFLUX, maps all eleven mammalian FG-nucleoporins within individual nuclear pores. The FG barrier is stratified rather than uniform: a wall-enriched central annulus surrounds a sparse axial corridor, dynamic open/closed caps sit at both pore faces, and Nup98 bridges the zones. Passive diffusion follows the central corridor while receptor-mediated transport follows FG-rich peripheral routes, motivating the Capped Adaptable Trichoid Channel (CATCH) model.

@article{yu_livecell_2026,
  title   = {Live-cell {3D} mapping reveals the organized architecture of {FG}-nucleoporins within the nuclear pore complex},
  author  = {Yu, Wenlan and Kelich, Joseph M. and Yu, Jingjie and Tingey, Mark and Li, Yichen and Junod, Samuel L. and Jiang, Zecheng and Hansen, Ian and Guessaymi, Nacef and Yang, Weidong},
  journal = {Nature Communications},
  year    = {2026},
  doi     = {10.1038/s41467-026-78936-5},
  url     = {https://doi.org/10.1038/s41467-026-78936-5},
  note    = {Article in Press}
}
@article{yu_livecell_2026, title = {Live-cell {3D} mapping reveals the organized architecture of {FG}-nucleoporins within the nuclear pore complex}, author = {Yu, Wenlan and Kelich, Joseph M. and Yu, Jingjie and Tingey, Mark and Li, Yichen and Junod, Samuel L. and Jiang, Zecheng and Hansen, Ian and Guessaymi, Nacef and Yang, Weidong}, journal = {Nature Communications}, year = {2026}, doi = {10.1038/s41467-026-78936-5}, url = {https://doi.org/10.1038/s41467-026-78936-5}, note = {Article in Press} }
Conference 2023

In vivo optogenetic stimulation using Parylene photonic waveguides for light delivery

Reddy J. W., Jain V., Guessaymi N., Malekoshoaraie M. H., Chamanzar M.

Proc. SPIE 12366, Optogenetics and Optical Manipulation 2023

DOI

Demonstration of flexible, biocompatible Parylene-C optical waveguides as implantable interfaces for in vivo optogenetic light delivery, presented at SPIE Photonics West.

@inproceedings{reddy_vivo_2023,
  title     = {In vivo optogenetic stimulation using Parylene photonic waveguides for light delivery},
  author    = {Reddy, Jay W. and Jain, Vishal and Guessaymi, Nacef and Malekoshoaraie, Mohammad H. and Chamanzar, Maysamreza},
  booktitle = {Optogenetics and Optical Manipulation 2023},
  year      = {2023},
  pages     = {14},
  publisher = {SPIE},
  doi       = {10.1117/12.2651613},
  url       = {https://doi.org/10.1117/12.2651613}
}
@inproceedings{reddy_vivo_2023, title = {In vivo optogenetic stimulation using Parylene photonic waveguides for light delivery}, author = {Reddy, Jay W. and Jain, Vishal and Guessaymi, Nacef and Malekoshoaraie, Mohammad H. and Chamanzar, Maysamreza}, booktitle = {Optogenetics and Optical Manipulation 2023}, year = {2023}, pages = {14}, publisher = {SPIE}, doi = {10.1117/12.2651613}, url = {https://doi.org/10.1117/12.2651613} }